Journal: ACS Omega
Article Title: Quantum-Dot-Based Enzyme Biosensor for Rapid Screening of Antibiotic Susceptibility in Urine Samples
doi: 10.1021/acsomega.5c07857
Figure Lengend Snippet: Fluorescence spectra of catalase-responsive antibiotic susceptibility testing (AST) using CdTe quantum dots excited at λ ex 420 nm. Spectra with (A) amoxicillin (Rresistant K. pneumoniae ATCC 700603, Ssensitive E. coli ATCC 25922), (B) nalidixic acid (Rresistant K. pneumoniae ATCC 700603, Ssensitive E. coli ATCC 25922), and (C) streptomycin (Rresistant Serratia marcescens ATCC 14756, Ssensitive K. pneumoniae ATCC 700603) as antibiotics to distinguish between resistant (R, green) and sensitive bacterial strains (S, blue) against the control (red, reaction components without catalase). Growth kinetics of bacterial strains treated with respective antibiotics are shown as insets. Additionally, F / F o values plotted against sensitive (blue) and resistant (red) strains are shown in the inset, as analyzed using GraphPad Prism software. P -values were calculated to assess the statistical significance, with values of P < 0.05 considered statistically significant.
Article Snippet: To evaluate the susceptibility of cultures ( K. pneumoniae ATCC 700603, E. coli ATCC 25922, Methicillin-resistant S. aureus (MRSA) ATCC 43300, S. aureus ATCC 25923, and S. marcescens ATCC 14756) against amoxicillin, nalidixic acid, streptomycin, colistin, and kanamycin, growth kinetic assays were performed.
Techniques: Fluorescence, Control, Software